Journal: Discover Oncology
Article Title: Single-cell and immune-context integration identifies basement-membrane/metastasis signatures that sharpen bladder-cancer diagnosis and prognosis
doi: 10.1007/s12672-026-04440-3
Figure Lengend Snippet: Cell lineage and spatial distribution of DDR2 and SERPINF1 in BLCA. A UMAP plot of single-cell transcriptomes colored by cell type, highlighting major populations including epithelial cells, fibroblasts, endothelial cells, monocytes, B cells, and adipocytes. Both DDR2 and SERPINF1 were predominantly expressed in fibroblasts. B , C Spatial transcriptomics analysis. Left: cell type annotation map of normal tissue; right: spatial expression heatmaps of DDR2 and SERPINF1 in tumor tissue. D , E Representative multiplex immunofluorescence (Opal-TSA) images acquired from the same section and the same field (ROI), showing DAPI nuclear staining and α-SMA and CD68 signals, with DDR2 (D) or SERPINF1 (E) channels displayed separately for clarity; merged images support the spatial transcriptomic findings. Scale bar, 50 μm
Article Snippet: The primary antibodies used were DDR2 (Proteintech, 67126-1-Ig), SERPINF1 (Proteintech, 26045-1-AP), CD68 (Abcam, ab213363), and α-SMA (Abcam, ab124964).
Techniques: Single Cell, Spatial Transcriptomics, Expressing, Multiplex Assay, Immunofluorescence, Staining